nmz787 | azonenberg: have you heard of these http://en.wikipedia.org/wiki/ISFET | 12:58 |
---|---|---|
Sync | nmz787: we are making them | 13:06 |
Sync | :) | 13:06 |
nmz787 | Sync: are there commercial/available-from-boutique-companies ISFETs that can detect single molecule changes? | 13:08 |
Sync | no | 13:09 |
Sync | or rather not that I know of | 13:09 |
nmz787 | so that must be why ion torrent seqeuncing still can't handle homopolymer stretches | 13:09 |
nmz787 | I'm looking for some method of detecting single molecule changes to a growing strand of DNA | 13:09 |
Sync | ew | 13:10 |
Sync | :D | 13:10 |
nmz787 | ahh. "The oblong design of the microchip contains millions of wells occupied by beads containing amplified copies of a single DNA strand. "..... from http://atp.ncifcrf.gov/genetics-and-genomics/laboratory-of-molecular-technology/lmt-technology-development/ion-torrent/ | 13:12 |
Sync | pretty happy that I repaired the VG mbe again | 13:12 |
nmz787 | I may have to seriously consider surface enhanced raman spectroscopy | 13:12 |
Sync | raman is nice | 13:13 |
Sync | but it is pretty amazing what shitty build quality you get when you buy vacuum equipment | 13:14 |
Sync | the vacuum side is usually very nice but the electronics on our evap are just .... sad | 13:14 |
nmz787 | what's VG mde? | 13:17 |
nmz787 | s/mde/mbe/ | 13:17 |
Sync | http://www.oxfordplasma.de/systems/v100_mbe.htm | 13:17 |
nmz787 | are you a microE by day? | 13:23 |
Sync | no, not really | 13:24 |
nmz787 | so you've got that MBE in your own lab? | 13:31 |
Sync | haha I wish, I work at the university as a side job | 13:31 |
Sync | wee my small oven works :) | 13:46 |
nmz787 | sounds like a nice side job! | 13:54 |
Sync | yeah it is pretty cool | 13:55 |
nmz787 | so Helicos is about 1 DNA molecule per sq micron on the hybridization surface | 14:48 |
nmz787 | http://www.helicosbio.com/Portals/0/Videos/tSMS-How_It_Works.flv | 14:50 |
--- Fri Aug 24 2012 | 00:00 |
Generated by irclog2html.py 2.9.2 by Marius Gedminas - find it at mg.pov.lt!